DZNep causes selective apoptosis in activated CD8⁺ T cells in vitro. (A-C) CD8⁺ T_N cells derived from B6 mice were stimulated with anti-CD3 and anti-CD28 Abs. Five days later, these activated T cells were further treated with different doses of DZNep for 24 hours. Live cell number was counted, and the recovery rate (output/input) was calculated (A). T-cell lysates were prepared for Western blot analysis to determine the amount of cleaved PARP (B). Dot plots and graphs (mean ± SD) show the fraction of annexin V⁺ cells in activated T cells treated with or without DZNep (200nM; C). (D-E) CD8⁺ T_N cells derived from B6 mice were cultured in the presence of IL-7 or IL-2 with or without the addition of DZNep (200nM) for 24 hours. CD8⁺ T cells that had been stimulated with anti-CD3 + anti-CD28 Abs for 5 days were collected and cultured with or without DZNep (200nM) for an additional 24 hours as controls. (D) Graphs (mean ± SD) show the percentage of dead cells after culture. (E) Western blots show the expressions of cleaved PARP. Representative results from ≥ 3 separate experiments are shown.