Shc is required for integrin-mediated spreading and haptotaxis on FN but not CL. (A) Shc is required for cell spreading on FN. Equal numbers of lentivirus-infected HUVECs were seeded on coverslips coated with 10 μg/mL of FN, 10 μg/mL of CL, or vehicle (PBS) and allowed to spread for 25 minutes. The cell area was measured using ImageJ software. Values shown are means ± SEM (n = 2 independent experiments, > 100 cells counted per condition per experiment). (B) Haptotaxis was measured using Boyden chambers coated on the underside with 10 μg/mL of FN, 10 μg/mL of CL, or vehicle (PBS) and blocked with 3% BSA. Cells that had migrated to the underside of the chamber were counted using an inverted microscope. Five random fields were imaged per filter. Values shown are means ± SEM (n = 3 independent experiments, 2 filters per condition per experiment). *P < .05