Shc is required for intersegmental vessel sprouting angiogenesis in zebrafish. (A) Shc protein depletion in Tg(kdrl:egfp) zebrafish embryos results in defective angiogenesis 30 hours after fertilization. Representative images of trunk vasculature are shown, with anterior on the left. Shc-MO fish exhibited a range of vascular phenotypes, the most common being delayed or defective intersomitic vessel sprouting and growth dorsally from the aorta. Scale bars indicate 200 μm. (B) Quantification of phenotypes observed in all living fish at 30 hours after fertilization displayed as a percentage of total. Control 4× MO, n = 118; Shc 2× MO, n = 72; and Shc 4× MO, n = 105 embryos. Numbers indicate all fish counted from 3 independent experiments.