Suppression of CD69 and CD154 expression on Tresps by ex vivo expanded Tregs. (A-B) Representative examples of CD69 and CD154 expression on Tresps either cultured alone (A) or cocultured with ex vivo expanded Tregs (B, here at a 1:1 Tresp:Treg ratio). (C-E) Percentage suppression of CD69 and CD154 expression on Tresps by Tregs, showing a representative experiment (C) and box-and-whisker plots of pooled data from 8 independent experiments (D-E). Please note that the regression line for CD69 extends only from the 8:1 to 1:1 conditions as suppression was barely visible below the 4:1 ratio. **P < .01. †P < .05, ††P < .001, †††P < .0001 with respect to Tresp alone cultures (Tresp:Treg ratio of 1:0).