Suppression of CD69 and CD154 expression on Tresps by autologous freshly isolated Tregs. (A-B) Representative examples of CD69 and CD154 expression on Tresps either cultured alone (A) or cocultured with Tregs (B, here at a 1:1 Tresp:Treg ratio). (C-D) Box-and-whisker plots of pooled data from 10 independent experiments. (E,G) Percentage suppression of CD69 and CD154 on Tresps by Tregs showing a representative experiment (E) and box-and-whisker plots (F-G) of pooled data from 10 independent experiments. *P < .05, **P < .01, ***P < .001. †P < .05, ††P < .001 with respect to Tresp alone cultures (Tresp:Treg ratio of 1:0).