Induction of profound thrombocytopenia by mAb 1B5 injection reduces plasma TGF-β1 levels in mice. WT C57BL/6 mice were injected intraperitoneally with the indicated amount of hamster mAb 1B5 against mouse αIIbβ3. (A) Platelet counts at the indicated time points after injection of 1B5. (B) Total TGF-β1 levels in plasma samples at the indicated time points as measured by ELISA. Each dot represents an individual mouse, and the different colors indicate samples from different mice. (C) Bleeding over the thorax and abdomen 24 hours after the 1B5 injection.