SIRT1 inhibition suppresses growth and induces apoptosis of BCR-ABL–transduced CD34⁺ cells. (A) Apoptosis of MIG210- or MIGR1-transduced normal CD34⁺ cells with mock (CtrlSh) or SIRT1 (shRNA) knockdown. The double-transduced CD34⁺ cells were sorted and then analyzed for annexin V⁺ cells after 48 hours of culture in low concentrations of growth factors similar to those present in long-term BM culture stroma-conditioned medium. (B-C) Analysis of total cell numbers (B) and CFC formation (C) of MIG210-transduced normal CD34⁺ cells with mock or SIRT1 knockdown. *P < .05; **P < .01. (D) Increased acetylation of FOXO1 and p53 after SIRT1 knockdown in MIG210-transduced normal CD34⁺ cells.