Platelet translocation pattern on immobilized wtD′A3 and T1255-, Clus1-, and DC-D′A3 glycosylation variants. Purified platelets were perfused over slides with D′A3 fragments immobilized at a concentration of 30 μg/mL at shear rate of 1000 s⁻¹ for 4 minutes. Thirty-five subsequent frames were superimposed (∼ 1.75 seconds) to track the translocation of individual platelets. Examples of platelets translocation paths on wt and mutated proteins are shown. When platelets jump rather than roll larger gaps between points of binding are seen; whereas when platelet roll uniformly and bind frequently no gaps are observed. Images were acquired at ×20 magnification and analyzed following ×3 zoom using ImageJ.