Polarized membrane domains are functionally important for homing and engraftment. (A-B) CD82 immunofluorescence labeling of human MPB CD34⁺ cells exposed to MβCD vehicle alone (A) or 10mM MβCD (B). (C) Percentage of human cell homing in NSG mice 16 hours after transplantation of control or MβCD-treated CD34⁺ cells (Donor 1 ▴, Donor 2 ●, Donor 3 ■, t test). (D) Percentage of human cell engraftment in NSG mice 2 months after transplantation of control or MβCD-treated CD34⁺ cells (t test). (E) Percentage of human cell homing in NSG mice 16 hours after transplantation of human CD34⁺ cells treated with isotype control or CD82 antibodies (Donor 1 ▴, Donor 2 ●, t test). (F) Percentage of human cells incubated with isotype control or CD82 antibodies adhering to osteoblasts in vitro (n = 3 donors, t test). Horizontal bars in panels C, D, and E represent means. Scale bars in panels A and B equal 5 μm (*P < .05; **P < .00001).