Tissue-specific generation of pDCs or cDC subpopulations on day 7 after transfer. (A) CD45.2⁺CCR9⁻ pDC-like precursors were transferred into CD45.1⁺ steady-state mice intravenously and progeny were analyzed 7 days later by flow cytometry for the expression of BST2, CD11b, CD8α, and CD103 in BM, spleen, lung, and liver. Results of one representative of 3 experiments are shown. (B) The percentages of BST2⁺ pDCs and BST2⁻ cDCs within CD45.2⁺CD11c⁺MHCII⁺PI⁻ cells derived from transferred CCR9⁻ pDC-like precursors after 7 days in BM, spleen, lung, and liver (mean ± SD, n = 3). (C) cDC subset compositions (CD8α⁺CD11b⁻ cDCs, CD8α⁻CD11b⁺ cDCs, and double negative cDCs) in spleen and lung originating from CD45.2⁺CCR9⁻ pDC-like precursors 7 days after transfer are depicted (mean, n = 3).