Dll4^hiDC-T cells produced high levels of IFN-γ but have impaired capacity to expand in vivo. Dll4^hiDC-induced CD4⁺ T cells (1.0 × 10⁶) of B6/SJL origin (H-2^b, CD45.1) were transplanted with TCD-BM (5.0 × 10⁶) into lethally irradiated BALB/c mice (H-2^d, CD45.2). Equal numbers of unstimulated B6/SJL CD4⁺ T_Ns were transferred to lethally irradiated BALB/c mice as controls. At days 3, 6, and 12 after transplantation, donor T cells were isolated from the spleens, lymph nodes (LN), liver, intestinal epithelial lymphocytes (IEL), and lamina propria lymphocytes (LPL) of recipient mice (3 mice per group). (A) Graphs show the numbers of donor (CD45.1⁺) CD4 T cells recovered from the spleens of recipient BALB/c mice at the indicated time points. (B) Histogram shows Ki67 and CFSE in donor CD4⁺ T cells 6 days after in vivo transfer. (C) Plots and graphs show the percentage of early apoptotic donor CD4⁺ T cells that were annexin V positive. (D) Graphs show the percentages and numbers of donor CD4⁺ T cells producing IL-2 in the spleen. (E) Plots and the graph show the percentage of donor FoxP3-positive Treg cells prior to transfer and 6 days after transfer. (F) Dot plots and graphs show the percentages and numbers of donor CD4⁺ T cells producing IFN-γ, TNF-α, and IL-17 in the spleen at day 6 and day 12 after transplantation. (G) Graphs show the number of donor CD4⁺ T cells producing IFN-γ, TNF-α, and IL-17 in GVHD target organs at day 12 after transplantation. Data show mean ± SD. Representative data from 2 independent experiments are shown. *P < .05; **P < .01; ***P < .001.