Phosphatase role in PLC-γ and Akt phosphorylation. (A) Dose-response analysis for PLC-γ and Akt phosphorylation. LC ECs were treated as described in Figure 3 in the presence or absence of Na₃VO₄ or Endothall. (B,D,F) Dose-response analysis of PLC-γ and Akt activation as a function of pY1054-VEGFR-2. Symbols and solid lines represent experimental data (symbols) and model (equations 18 and 34 in supplemental Sections 1.3 and 1.4). PLC-γ and Akt phosphorylation, respectively, are shown for cells treated with Na₃VO₄ (B) and with either Na₃VO₄ (D) or Endothall (F). (C,E,G) Dose-response analysis of PLC-γ and Akt phosphorylation as a function of VEGF concentration. Symbols and solid lines represent experimental data and model (equations 19 and 35 in supplemental Sections 1.3 and 1.4). PLC-γ is shown for cells treated with Na₃VO₄ (C) and Akt phosphorylation for cells treated with either Na₃VO₄ (E) or Endothall (G). Experimental values of pY1054-VEGFR-2 and phosphorylated PLC-γ (pPLC-γ) were obtained using vinculin signal as normalizer, whereas experimental values of phosphorylated Akt (pAkt) were obtained using tubulin signal. Values are the mean ± SD of 3 experiments.