LMO2 interacting proteins in DLBCL. (A-B) Protein expression in nuclear extracts of DLBCL cell lines was assayed by immunoblotting with specific antibodies. Immunoblotting for actin and GAPDH served as a loading control. (C) Nuclear extracts were prepared from the SU-DHL-6 and OCILy19 cell lines and used for immunoprecipitation of indicated proteins and immunoblotting with LMO2 antibody. (D) A table summarizing the immunoprecipitation results shown in supplemental Figure 2. Coimmunoprecipitation in at least one cell line was defined as positive interaction. (E) Nuclear extracts were prepared from the SU-DHL-6 and OCILy19 cell lines and used for immunoprecipitation with LMO2 antibody and immunoblotting with SP1 antibody. (F) Nuclear extracts were prepared from the SU-DHL-6 cell line and used for immunoprecipitation of indicated proteins and immunoblotting with SP1 antibody. Results in panels A through F are representative of 3 independent experiments.