nTregs and iTregs contribute to protection against GVHD-induced weight loss. CD45.1⁺ FoxP3.GFP or Scurfy BM was mixed with CD45.2⁺ B6 BM at a 2:1 ratio and injected into irradiated B6 mice. CD45.1⁺ GFP⁺ and GFP⁻ T cells were sorted from the mixed BM chimeras. The T cells were then used as effectors for GVHD induction by mixing the T cells in the following combinations: nTregs + iTregs (0.1 × 10⁶ GFP⁺ Tregs + 0.5 × 10⁶ GFP⁻ Tconvs), nTregs alone (0.1 × 10⁶ GFP⁺ Tregs + 0.5 × 10⁶ Scurfy Tconvs), iTregs alone (0.5 × 10⁶ GFP⁻ Tconvs), and no Tregs (0.5 × 10⁶ Scurfy Tconvs). Weight changes were monitored over time. Two independent experiments are shown. The results are represented as the percent mean weight change ± SEM (n = 10 mice/group for experiment I and n = 5 mice/group in experiment II). *P < .05 by ANOVA compared with the group receiving no Tregs (Scurfy Tconvs alone).