JNK2 phosphorylation levels are increased in platelets and thrombi in diabetic mice. (A) Washed platelets from wt or cd36-null mice were treated with AGE-BSA or BSA control (50 μg/mL) for 15 minutes. Lysates were then assessed by immunoblot using anti–phospho-JNK2 Ab. An Ab to total JNK2 was used as a loading control. Blot is representative of 4. (B) Platelets from chow-, DBD-, and STZ-fed wt or cd36-null mice were analyzed by immunoblot as in panel A. A representative immunoblot of 4 is shown. The bar graph shows the ratio of phospho-JNK2 (p-JNK2) to total JNK2. (C) Representative images of thrombosed carotid arteries from chow-, DBD-, and STZ-fed wt or cd36-null mice stained with anti–p-JNK. Bar graph shows staining intensity scores for p-JNK (n = 5 mice per group).