AGE-BSA enhances platelet aggregation in a CD36-dependent manner. (A) Platelets were incubated with increasing concentrations of AGE-BSA for 30 minutes and then assessed for aggregation in response to low-dose ADP (1μM). On the left are representative tracings from n = 6, and on the right a bar graph showing mean amplitudes of aggregation expressed as the percentage of light transmission using control PPP (± SEM). (B) Platelets from wt or cd36-null mice were incubated with 100 μg/mL of AGE-BSA or BSA and then analyzed as in panel A (n = 4). On the right is a bar graph showing mean amplitudes of aggregation (± SEM). (C) PRP obtained from healthy donors was preincubated with 50μM of AGE-BSA or BSA control for 30 minutes and then stimulated with 5μM ADP. The left panel shows representative tracings; the right panel is a bar graph showing the mean amplitudes of aggregation (± SEM) of 3 independent experiments.