RAGE and other potential AGE receptors are expressed on mouse platelets. (A) Platelet lysates from wt and cd36-null mice were subjected to immunoblot assays with the indicated Abs. Membranes were stripped and reblotted with antiactin as a loading control. Band densities were measured and plotted as ratios to actin (n = 3). (B) Isolated wt platelets were incubated with 10 μg/mL of RAGE-blocking Ab or control IgG 45 minutes before being treated with biotin-labeled AGE-BSA, as described in Figure 1A. The histogram shown is representative of 4 and the bar graph shows mean fluorescence intensities (± SEM).