Setbp1 directly activates Hoxa9 and Hoxa10 transcription. (A) Amino acid sequence alignments of AT-hook DNA-binding motifs present in human and mouse Setbp1 protein. Amino acid numbers are indicated. The invariable consensus sequence of AT-hook motifs (RGRP) are highlighted in gray. (B-C) ChIP analysis of myeloid progenitors immortalized by the expression of 3xFLAG-tagged Setbp1 using anti-FLAG M2 antibody and control IgG. PCR products using primers specific to various regions of Hoxa9 (B) or Hoxa10 locus (C) were resolved on ethidium bromide-stained agarose gel (top panel). Results are representative of 3 independent experiments. Diagrams of tested region of Hoxa9 and Hoxa10 locus are also shown (bottom panels). Locations of PCR amplicons are indicated as black bars with corresponding numbers. Transcriptional start sites are indicated as arrows. Exons are indicated as white boxes with coding regions highlighted in black.