Altered expression of factors promoting B lymphopoiesis and osteoblastoid differentiation in Smo-depleted OP9 cells. (A) Volcano plot (left) and Venn diagram (right) of differentially expressed and functionally clustered transcripts in Smo-depleted OP9 cells. In the volcano plot, statistical significance (determined by 1-way ANOVA) is graphed as a function of the fold change (control/Smo-depleted). Transcripts with known roles in hematopoiesis, osteoblastoid differentiation, or both are designated on the Volcano plot and identified in the Venn diagram. Transcripts encoding ECM proteins or cell adhesion proteins are designated by blue and red dots, respectively. Black dots represent transcripts encoding proteins that fall into both functional categories. Red circles designate transcripts with changes in expression verified by quantitative RT-PCR. Transcripts corresponding to each functional set are listed. (B) Validation of array results. Indicated transcripts in control or Smo-depleted (Smo kd) cells were quantified by RT-PCR and amounts were normalized to Actb. Normalized values for Smo-depleted cells are expressed relative to the corresponding values for control cells (mean and SD, n = 3).