Antilymphoma effect of combined inhibition of eIF4E and TEHsp90. (A) Effect on BCL6, BCL2, MYC, Hsp70, Hsp90, and actin (as control) protein abundance of vehicle, ribavirin (RBV), PU-H71, and their combination (combo) in SU-DHL6 (SU6), DoHH2 (Do2), and OCI-Ly1 (Ly1) cells. Densitometry analysis is shown on the right for triplicate experiments as mean ± SEM. C, combination; P, PU-H71; R, ribavirin; V, vehicle. (B) BCL6, MYC, BCL2, and GAPDH polysomal profiling of OCI-Ly1, DoHH2, and SU-DHL6 cells treated with vehicle (black dotted line) or the TEHsp90 inhibitor PU-H71. Each profiling is normalized to its respective vehicle represented with a dotted central line. Points localized below the line represent polysome fractions depleted in PU-H71-treated cells. (C-D) Tumor growth (in AUC from day 1 to day 10) of OCI-Ly1 and SU-DHL6 xenografted mice. P values are shown on top for each group for the comparison with vehicle-treated mice. (E) Tumor growth (in AUC from day 1 to day 20) of TH DLBCL PDX xenografted mice. P values are shown on top for each group for the comparison with vehicle-treated mice. (F) Body weight at day 21 of mice from panel E.