eIF4E is expressed in DH/TH DLBCL. (A) Representative image of BCL2, BCL6, and MYC FISH assay carried out in OCI-Ly1, SU-DHL6, and DoHH2 cell lines. Summary of FISH findings for MYC, BCL2, and/or BCL6 in 6 DLBCL cell lines harboring >1 of such chromosomal abnormalities. (B) Scatter plot for PU-H71 GI₅₀ (growth inhibitory concentration 50%) in DH/TH cell lines (n = 6) vs non-DH/TH DLBCL cell lines (n = 29). (C) eIF4E expression by immunohistochemistry from 111 DLBCL cases. Number of positive cells (over total cells) is represented by color scale. All the DH/TH cases in the cohort (n = 6) presented the highest levels of eIF4E staining (n = 10). (D) Affinity purification of eIF4E and TEHsp90 from DH/TH cell lines nuclear lysates using PU-H71-beads vs chemical control beads.