Intravenous transplantation of CD11b+ KC in C57Bl/6 hemophilia mice. (A) Percent survival in mice after tail-clip bleeding. All untreated control mice died, as did mice 3 days after transplantation of 2.5 to 3 × 106 KCs from hemophilia A donors, and mice 3 days after transplantation of 1 × 105 LSECs from C57Bl/6 mice (n = 6 ea). In recipients of 2.5 to 3 × 106 KCs from healthy C57Bl/6 GFP donors, tail-clip after 3 days and 7 days produced survival (P = .02 and 0.07, respectively; Fisher exact test). (B) Localization of transplanted KCs in liver by immunostaining for F4/80 (KC marker, red) and GFP (transplanted cell marker, green). F4/80+ KCs in liver sinusoids 3 days after KC transplantation in hemophilia mouse (arrowhead). A transplanted KC is shown with GFP plus F4/80 (arrow). (C) Shows CD11b+ peripheral blood MNCs from WT and hemophilia A mice with staining for CD14 and FVIII. Original magnification (B) ×630, (C) ×400.
