Cell-autonomous requirement for agrin in myeloid cell development. (A) Annexin V analysis on monocytes and macrophages of donor origin determined 9 weeks after BM transfer (ctrl, n = 4; Musk-L;Agrn^−/−, n = 4). (B) Analyses of donor monocytic cells in BM, PB, and spleen 9 weeks after BM transfer (n = 4), and after a second serial (9 weeks plus 9 weeks) BM transfer (n = 6). In all panels, error bars represent SEM and *P ≤ .05, **P ≤ .01.