IKKα inactivation deregulates the expression of genes involved in BM B-cell development. (A) Heat map and hierarchical cluster analysis expression of 36 genes (supplemental Table 2) in WT and KA/KA BM B220⁺ cells, as identified using microarray analysis. Red and blue indicate up-regulated and down-regulated genes, respectively. Standardized intensity is indicated by the fold values (Log2) of gene expression in WT and KA/KA BM B cells. (B) Western blot showing IRF4 and Pax5 levels in B220⁺-enriched cells, B220-flow, and splenocyte lysates (see Figure 5A). β-actin, protein-loading control (same as in Figure 5A). (C) Expression of Pax5, IRF4, and IKKα analyzed with RT-PCR on serially diluted total RNA isolated from the BM B220⁺ cells of WT and KA/KA mice. ++, +, cDNA dilution; -, negative control; β-actin, PCR control. (D) IRF4 and Pax5 levels in the BM B220⁺ cells of WT (+/+) and KA/KA mice detected with Western blot. α-tubulin, protein-loading control. (E) Stat3 activity (p-Stat3) and Ikaros level in the BM B220⁺ cells of WT and KA/KA mice. β-actin, protein-loading control.