CD8 T-cell clone 2196.D11_b exhibits specific cytotoxic activity for cyclin-A1–expressing primary AML samples. (A) Four-hour caspase-3 assay. Specific caspase-3 cleavage is shown after subtraction of spontaneous caspase-3 activation, and determined relative to staurosporine-induced cleavage as 100%. (B) Four-hour ⁵¹Cr release assay at a range of E:T ratio with the same effector and target cells. H001 and 1690-59 are HLA A*0201 positive, and R10009 and R50400 are A*0201-negative.