HSCs generated by HoxB4 overexpression have impaired lymphoid-lineage gene-expression pattern. We examined genes up-regulated in 2 subtypes of HSCs, myeloid-biased and lymphoid-biased, described previously by Challen et al referred to herein as lineage-biased genes.⁴²  Normalized expression of lineage-biased genes from our study and Challen et al was compared. (A) Overall expression correlation of lineage-biased genes between various developing and mature HSCs. D0, D6, D16, and D26 refer to day 0, 6, 16, and 26 cells from this study, respectively. (B) Normalized expression levels of myeloid-lineage-biased genes (ie, up-regulated in My-HSCs identified by Challen et al⁴² ) in ESC-derived HSCs (day 26 cells from the present study), My-HSCs, and Ly-HSCs. (C) Normalized expression level of lymphoid-lineage-biased genes (up-regulated in Ly-HSCs identified by Challen et al⁴² ) in ESC-derived HSCs (day 26 cells from the present study), My-HSCs, and Ly-HSCs. P values are from 1-tailed t tests.