Binding site overlap between HoxB4 and a set of 10 hematopoiesis TFs. ChIP-Seq peaks for the set of hematopoiesis TFs were described previously.37 (A) Number of binding peaks and significance of binding-peak overlap between HoxB4 and the 10 TFs. Significance is shown as the minus logarithm of P value for binding peak overlap. P values were calculated using a permutation test (for details, see supplemental Methods). Overlapping TFs with P < .001 are highlighted in red. (B) Box plot of peak center distance between HoxB4 binding peaks and peaks of other TFs. (C) Breakdown of different classes of overlapping peaks involving the 4 significantly overlapping TFs: H, HoxB4; F, Fli1; M, Meis1; R, Runx1; and S, Scl. Class names are based on the combination of different TFs present in the binding peaks. (D) Gene ontology term enrichment analysis of different classes of combinatorial peaks. Red indicates a gene ontology term enriched at FDR = 0.05; black, gene ontology term not enriched. Dendrogram was clustered using hierarchical clustering.
