Gene-expression profiles during ESC differentiation into hematopoietic cells. (A) Expression profiles of classic genes important for ESC and HSC phenotypes. Solid line is ESC genes; dashed line, HSC genes. (B) Overlap between differentially expressed and differentially bound genes between 2 adjacent time points. Differential binding means that the gene is only bound by HoxB4 at 1 of the 2 time points. Top, schematic illustrating the 2 sets of genes and their overlap. T_i and T_i+1 indicate adjacent time points; ●, HoxB4 protein. Bar height represents the expression levels of the target genes; Bottom, for adjacent time points, the second number represents the number of differentially expressed genes and the first number represents the number of differentially expressed genes that overlap with differentially bound genes.