Effects of RHAU ablation on progenitors and stem cells. (A) FACS analysis of MEP, GMP, and CMP cells in BM and spleen. Lin⁻CD127Ra/IL-7Ra⁻ living cells were gated and analyzed for c-kit⁺ and Sca-1⁻ populations (LK). The LK subgroup was further gated for CD34 and CD16/32 (FcγR). CMPs, GMPs, and MEPs were identified as FcγR^lowCD34⁺, FcγR^highCD34⁺, and FcγR^lowCD34⁻, respectively. The mean proportions ± SEs of CMP, GMP, and MEP cells are indicated in the graphs. *P < .05; n = 6. (B) Absolute numbers of progenitor cells in the BM and spleen; *P < .05; n = 6. (C) FACS analysis of LT-HST, ST-HST and LMPP cells in BM. Lineage-negative BM cells were first gated to obtain IL-7Ra⁻Sca-1⁺c-Kit⁺ cells, which were further gated into 2 populations: Thy1.1^lo/+ and Thy1.1⁻. These were further probed for VCAM-1 and Flt3/CD135 expression. LT-HSCs, ST-HSCs, and LMPPs were defined as Thy1.1⁺VCAM-1⁺Flt3⁻, Thy1.1⁺VCAM-1⁺Flt3⁺, and Thy1.1⁻VCAM-1⁻flt3⁺, respectively. The mean proportions ± SEs of different populations are indicated in the graphs; *P < .05; n = 6. (D) Effect of RHAU ablation on the numbers of stem cells. The mean numbers ± SEs of each cell type are shown as bar charts; *P < .05; n = 6.