Figure 4
Figure 4. Differential effects of RHAU ablation on erythroid cell differentiation in the BM and spleen. (A-B) The different developmental stages were identified by FACS with the use of Ter-119 and CD71 markers together with the forward scatter (FSC) parameter.28 Ter119+ cells are classified into the following 4 subgroups: ProE (Ter119med CD71hi), Ery.A (Ter119hiCD71hiFSChi), Ery.B (Ter119hiCD71hiFSClow), and Ery.C (Ter119hiCD71lowFSClow). The mean proportions of each subgroup ± SEs (n = 4) are shown beside the boxes in the dot plots. (C) Effect of RHAU ablation on total numbers of erythroblasts in the BM and spleen. Cell numbers of the 4 subgroups are indicated in the bars by different colors, denoting, from the top, Pro.E, Ery.A, Ery.B, and Ery.C. *P < .05; n = 4.

Differential effects of RHAU ablation on erythroid cell differentiation in the BM and spleen. (A-B) The different developmental stages were identified by FACS with the use of Ter-119 and CD71 markers together with the forward scatter (FSC) parameter.28  Ter119+ cells are classified into the following 4 subgroups: ProE (Ter119med CD71hi), Ery.A (Ter119hiCD71hiFSChi), Ery.B (Ter119hiCD71hiFSClow), and Ery.C (Ter119hiCD71lowFSClow). The mean proportions of each subgroup ± SEs (n = 4) are shown beside the boxes in the dot plots. (C) Effect of RHAU ablation on total numbers of erythroblasts in the BM and spleen. Cell numbers of the 4 subgroups are indicated in the bars by different colors, denoting, from the top, Pro.E, Ery.A, Ery.B, and Ery.C. *P < .05; n = 4.

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