Phenotypes of RHAU^fl/fl:vav-iCre mice. (A) Anemic appearance of newborn RHAU^fl/fl:vav-iCre mice with specific RHAU ablation in the hematopoietic system. (B left) Western blot (WB) analysis of the efficiency of RHAU ablation in hematopoietic organs. (B right) Genotype analysis of HSCs by PCR. HSCs were sorted by FACS as described in “FACS analysis” (supplemental Table 1). The genotypes of mice are (1), RHAU^fl/fl; (2), RHAU^fl/+:vav-iCre; and (3), RHAU^fl/fl:vav-iCre. (C) Liver, spleen, thymus, and kidney of RHAU^fl/fl:vav-iCre and control RHAU^fl/fl mice. (D) Total body mass and relative body mass of each organ from RHAU^fl/fl:vav-iCre and RHAU^fl/fl mice. Each dot represents the relative body mass expressed as the ratio of organ weight to total body weight. *P < .05; n = 16. (E) H&E staining of spleen and thymus from RHAU^fl/fl and RHAU^fl/fl:vav-iCre mice (magnification, ×4; bar, 500 μm). RHAU^fl/fl and RHAU^fl/fl:vav-iCre are presented as fl/fl and fl/fl:iCre in figures, respectively.