DOCK8 regulates DC trafficking to the draining LNs during immune responses. (A) Uptake of FITC-OVA and FITC-dextran were compared at 37°C or 4°C between Dock8^+/− and Dock8^−/− BM-derived DCs. (B) After fixation with 1% paraformaldehyde, OVA protein-pulsed Dock8^+/− and Dock8^−/− DCs were cultured with CD4⁺ OT-II T cells. (C) Migration of epidermal DCs was induced by applying 200 μL of 1% FITC isomer I onto the shaved abdomen. The frequency of FITC⁺ MHC class II⁺ DCs was compared between Dock8^+/− and Dock8^−/− mice. (D) The migration efficiency of Dock8^+/− and Dock8^−/− DCs to the popliteal LNs was compared 48 hours after injection into footpads of C57BL/6 mice at a 1:1 ratio (n = 3 mice per group). Before assays, DCs were stimulated with or without LPS (200 ng/mL). Data are expressed as means ± SD with representative flow cytometric profiles. **P < .01. Data are representative of 2 (A-B), 4 (C), or 3 (D) independent experiments.