Effect of DOCK8 deficiency on leukocyte subsets under the steady state. (A-B) Splenocytes (A; n = 10 mice per group) and peripheral LN cells (B; n = 8 mice per group) from 6- to 7-week-old mice were analyzed for the expression of CD4 and CD8 or Mac1 and B220. The numerals in quadrants indicate the percentage of cells in each. The number of each subset of cells was compared between Dock8^+/− and Dock8^−/− mice. **P < .01. (C) Dock8^+/− and Dock8^−/− T cells were labeled with different fluorescent dyes and IV injected into C57BL/6 mice at a 1:1 ratio. T-cell homing was analyzed 1 hour after transfer (n = 3 mice per group). Localization of Dock8^+/− (red) and Dock8^−/− (green) T cells in the spleen was compared 48 hours after transfer. Metallophilic macrophages (blue) were stained with MOMA1 Ab. Scale bar indicates 100 μm. (D) Splenic B220⁺ B cells were analyzed for the expression of CD21 and CD23 at the age of 10 weeks. The number of CD21⁺CD23⁻ MZB cells was compared between Dock8^+/− and Dock8^−/− mice (n = 4 mice per group). **P < .01. (E) Serum concentrations of IgM, IgG1, IgG2b, and IgE were compared between 7- to 12-week-old Dock8^+/− and Dock8^−/− mice (n = 10-11 mice per group). Each symbol represents an individual mouse; small horizontal lines indicate the average. Data are from 9 (A), 8 (B), 3 (C), or 2 (D-E) separate experiments and are expressed as means ± SD with representative flow cytometric profiles.