Figure 4
![Figure 4. Increase of F-actin stress fibers by treatment with ephrinB2-specific scFvs. (A) Detection of polymerized actin was performed by staining with Alexa 568–labeled phalloidin on HUVEC cultures treated with B11, 2B1, or irrelevant scFv (C-) for 4 hours. (B) Cell area (μm2) and (C) fluorescence intensity (arbitrary fluorescence unit [AFU]) were quantified using Definiens Software XD Version 1.5 and were represented as the median of 150 cells (n = 150), upper and lower quartile, and upper and lower extreme for each group. *P < .001 versus control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/19/10.1182_blood-2011-09-380006/4/zh89991290600004.jpeg?Expires=1724250817&Signature=ikfJmkmOsYYpxDvMgUTCkixC12wDPgc3eyqX7yn1Sc5kv2-e2G6TPMN72Zxa4F5LsHKoSXKU20jWEClLDxYJBv-9t-5KvpGy6kQmy9f~Vx7OLalqxzjZBZ9ew5dbBW6u2MbV6DINu7tQAkXcvfi0SlRPnq2s5zUfdQDq2aIXNLWxdVaTGwrH7lvf4YdO5ENNs1JUiSXnw-1eBbjf3dnJcgxcz8PtC6sC5Cveifj78il47ElLqYyoMIqeerjDZqj6vqnNbV1hasDtAxBasGS-AsntRVRUrCIAV7CMObJJBBNRp~EGZFWJZW89p0dKabRWhPIW7zHk~4j2T95cfx3sBQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increase of F-actin stress fibers by treatment with ephrinB2-specific scFvs. (A) Detection of polymerized actin was performed by staining with Alexa 568–labeled phalloidin on HUVEC cultures treated with B11, 2B1, or irrelevant scFv (C-) for 4 hours. (B) Cell area (μm2) and (C) fluorescence intensity (arbitrary fluorescence unit [AFU]) were quantified using Definiens Software XD Version 1.5 and were represented as the median of 150 cells (n = 150), upper and lower quartile, and upper and lower extreme for each group. *P < .001 versus control.
