HMGB-1, IL-8, and IL-8 receptor (CXCR1 and CXCR2) expression by ALK⁺ALCL patients. HMGB-1 (A) or CXCR1 and CXCR2 (C) mRNAs levels were evaluated by qRT-PCR on 15 ALK⁺ALCL biopsies. mRNA amounts were normalized to levels found in reactive lymph nodes. Results are expressed as the mean ± SD of independent experiments done in triplicate. **P < .01 by Student t test. (B,E) HMGB1 and IL-8 secretion was measured using an ELISA assay on sera from 35 ALK⁺ALCL patients with (n = 20) or without (n = 7) leukemic dissemination and from healthy patients (n = 10). The number of biopsies in each group corresponds to the n value. (D) CXCR1 and CXCR2 immunohistochemical staining of lymph nodes from 2 ALK⁺ALCL patients. Sections were stained with anti-CXCR1 or anti-CXCR2 Abs, and nuclei were counterstained with hematoxylin. Vessels were positive for both CXCR staining and were used as an internal control (asterisks). Staining intensity varies from tumoral cell to cell. Original magnification, 200×.