The effect of dasatinib on neutrophil migration. (A-B) Human neutrophils pretreated with the indicated concentrations of dasatinib were allowed to migrate toward 10μM fMLP (A) or 1 μg/mL of human IL-8 (B) on a fibrinogen-coated coverslip in a Zigmond chamber assay. Representative single-cell tracks, rose diagrams of direction of movement, and the results of quantitative analysis are shown. Bar graphs show mean and SD of representative experiments, and the dose-response curves show mean and SEM of the percent response from 263-476 cells from 3-7 independent experiments. (C-D) Adhesion (C) and migration (D) of neutrophils in the presence of 1μM fMLP in a flow chamber assay (all samples were treated with fMLP). Panel C shows the number of adherent cells per field of view (FOV). Panel D shows migration characteristics of cells that remained adherent through the entire 10-minute flow period. Data in panels C and D show mean and SEM from 5 independent experiments. (E-F) Cells were allowed to migrate toward 100nM fMLP or 10 ng/mL of IL-8 through FCS-coated Transwell filters (E) or a Matrigel matrix (F). Data in panels E and F show mean and SEM from 4-5 independent experiments.