Multimeric analysis of expressed VWF. VWF in the conditioned medium of transiently transfected HEK293T cells was analyzed by electrophoresis in 2% SDS-agarose gels and immunoblotted as described in “Multimer analysis.” Panels A and B represent 2 individual gels. In panel B, lanes were removed for clarity as shown by the separation. (A) Multimeric structure of WT-VWF (lane 1), M740I-VWF (lane 2), M740I/C1190S-VWF (lane 3), and C1190S-VWF (lane 4). (B) Multimeric composition of WT-VWF (lane 1); cysteine mutations C1272S-VWF, C1272Y-VWF, and C1099P-VWF (lanes 2-5); I1568N-VWF (lane 6); G1579R-VWF (lane 7); and G1631D-VWF (lane 8). VWF variants involving mutation of cysteines results in abnormal multimer structure.