Figure 3
Figure 3. Mice undergoing Ott1 deletion are able to maintain peripheral blood counts and BM HSC populations older than 18 months but have reduced colony-forming potential. (A) Automated peripheral blood analysis of samples from 18-month-old Ott1flox/null Mx1-cre (KO) or littermates possessing a WT Ott1 allele (control) pIpC-treated at 6 weeks of age. Control, n = 6; KO, n = 5. (B) Absolute numbers of LT-HSCs (Lin−Sca-1+c-Kit+CD34−), ST-HSCs (Lin−Sca-1+c-Kit+CD34+), progenitors (Lin−Sca-1−c-Kit+), and total BM obtained from 18-month-old Ott1 KO and control mice in panel A. (C) Myeloid CFU ability of BM from 18-month-old Ott1 KO or control mice in panel A. Samples plated in duplicate (control, n = 4; Ott1 KO, n = 3). Gran indicates CFU-granulocyte; GM, CFU-granulocyte/monocyte; Mono, CFU-monocyte; and Ery, CFU-erythroid. Graphs represent mean values, and error bars represent SD.

Mice undergoing Ott1 deletion are able to maintain peripheral blood counts and BM HSC populations older than 18 months but have reduced colony-forming potential. (A) Automated peripheral blood analysis of samples from 18-month-old Ott1flox/null Mx1-cre (KO) or littermates possessing a WT Ott1 allele (control) pIpC-treated at 6 weeks of age. Control, n = 6; KO, n = 5. (B) Absolute numbers of LT-HSCs (Lin−Sca-1+c-Kit+CD34−), ST-HSCs (Lin−Sca-1+c-Kit+CD34+), progenitors (Lin−Sca-1−c-Kit+), and total BM obtained from 18-month-old Ott1 KO and control mice in panel A. (C) Myeloid CFU ability of BM from 18-month-old Ott1 KO or control mice in panel A. Samples plated in duplicate (control, n = 4; Ott1 KO, n = 3). Gran indicates CFU-granulocyte; GM, CFU-granulocyte/monocyte; Mono, CFU-monocyte; and Ery, CFU-erythroid. Graphs represent mean values, and error bars represent SD.

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