Cebpa-Cre–driven deletion of Dicer1 causes neutrophil dysplasia and monocyte/macrophage depletion in mice. (A) FACS analysis of BM neutrophils with Abs against CD11b and Ly-6G for Dicer1^wt/Δ (similar to Dicer1^wt) and Dicer1^Δ/Δ neutrophils. (B) Micrographs showing morphology of Dicer1^wt/Δ (similar to Dicer1^wt) and Dicer1^Δ/Δ neutrophils. Black bar indicates 10 μm. (C) Percentage of EYFP⁺ cells in the blood of Dicer1^wt/Δ and Dicer1^Δ/Δ mice. (D) Analysis of peripheral neutrophils with Abs against CD11b and Ly-6G. (E) Analysis of peripheral EYFP⁺ monocytes (CD11b⁺ or Ly-6G⁻) with Abs against Ly-6C. (F) Analysis of EYFP⁺ macrophages from the abdominal cavity with Abs against F4/80 Ag. (G) FACS analysis of total spleen cells with Abs against CD11b and Ly6G. All analyses were performed on at least 3 mice.