The role of Prmt1 in AE9a-mediated self-renewal in murine BM cells. (A) Western blot analysis for AE9a and Prmt1 expression from BM cells cotransduced with AE9a, control shRNA, or shPrmt1 retroviruses after puromycin selection. α-tubulin is used as a loading control. (B) Bar chart represents the number of colonies from the third-round replating of BM cells. Error bars represent SDs of 3 independent experiments. (C) BM cells were cotransduced with AE9a or E2A-PBX in combination with control shRNA or shPrmt1 #1. Bar chart represents the relative number of colonies from the third-round replating of BM cells. Error bars represent SDs of 2 independent experiments. (D) Typical image of the third-round colonies generated from BM cells cotransduced with AE9a, control shRNA, or shPrmt1 retroviruses. (E) Western blot analysis for Prmt1 expression from AE9a-leukemic cells transduced with control shRNA or shPrmt1 retroviruses after puromycin selection. α-tubulin is used as a loading control. Images were taken using Nikon Eclipse TS100 microscope with 10×/0.25 objective lens and Nikon DS Camera Control Unit DS-U2 system. (F) Bar chart represents the number of colonies from the second- and third-round replating of primary AE9a leukemic cells. Error bars represent SDs of 3 independent experiments.