Figure 5
Figure 5. ChIP analyses of AE9a, PRMT1, histone H4R3Me2a, and histone H3K9/14Ac on promoters of interest. (A) Schematic diagrams show promoters analyzed by ChIP. Positions of the predicted transcription initiation sites (solid arrows) are indicated. Relative positions of consensus AML1 sites (white boxes) and primers (dotted arrows) in each promoter are shown. (B) ChIP results for AE9a. (C) ChIP results for PRMT1. (D) ChIP results for H4R3Me2a. (E) ChIP results for H3K9/14Ac. Promoter of ACTB is used as an internal control. Data represented are relative enrichment of specific ChIP target in K562-AE9a cells over WT K562 cells. Error bars represent SDs of 3 independent experiments.

ChIP analyses of AE9a, PRMT1, histone H4R3Me2a, and histone H3K9/14Ac on promoters of interest. (A) Schematic diagrams show promoters analyzed by ChIP. Positions of the predicted transcription initiation sites (solid arrows) are indicated. Relative positions of consensus AML1 sites (white boxes) and primers (dotted arrows) in each promoter are shown. (B) ChIP results for AE9a. (C) ChIP results for PRMT1. (D) ChIP results for H4R3Me2a. (E) ChIP results for H3K9/14Ac. Promoter of ACTB is used as an internal control. Data represented are relative enrichment of specific ChIP target in K562-AE9a cells over WT K562 cells. Error bars represent SDs of 3 independent experiments.

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