RNAi-mediated suppression of GLUT8 or GLUT11 compromises the viability of myeloma cell lines. (A) Cells were transduced with the indicated shRNAs and incubated 2 days before protein extraction. Representative blot is shown. (B) Cells from panel A were cultured in 5mM glucose-containing medium for 5 hours. Glucose consumption rates and lactate production rates were determined and normalized to control shRNA-expressing cells. (C-E) Cells from panel A were analyzed for viability and proliferation. Viable cell densities are expressed as fold change relative to the day 0 reading of control shRNA-expressing cells. (F) GLUT8 subcellular localization in KMS11 cells, L363 cells, and NBLs was assessed via confocal immunofluorescence microscopy. Representative images are shown. (G) Cells were transduced with the indicated shRNAs and incubated 3 days before RNA extraction. (H) Cells from panel G were cultured in 5mM glucose-containing medium for 5 hours. Glucose consumption rates and lactate production rates were determined and normalized to control shRNA-expressing cells. (I-K) Cells from panel G were analyzed for viability and proliferation. Viable cell densities are expressed as fold change relative to the day 0 reading of control shRNA-expressing cells. (L) GLUT11 subcellular localization in KMS11 cells, L363 cells, and NBLs was assessed via confocal immunofluorescence microscopy. Background, nonspecific staining with preimmune serum is included as a control. Representative images are shown. (B-E,G-K) Data are mean ± SEM. (A-L) n ≥ 3. *P < .05. **P < .01. ***P < .005.