Effect of silencing NHERF-2 on in vivo tumorigenesis. (A) Efficient knockdown of NHERF-2 in bEnd.3 cells by lentiviral shRNA as determined by real-time PCR. (B) Average tumor volume (in mm3) in control shRNA- versus NHERF-2 shRNA-bearing groups. Ten mice were injected with 1 × 106 cells, and animals were killed on day 14. Statistical significance was determined with 2-sided Student t test, and *P < .05 was considered significant. (C) Histologic analysis by H&E staining found typical cavernous hemangiomas in control-shRNA and (D) NHERF-2 shRNA–bearing groups. Original magnification, ×20. (E) Ki67 staining of the control shRNA–bearing hemangioma. Arrows indicate lack of Ki67 (brown) staining; instead hematoxylin counter-stained blue nuclei are visible. (F) Ki67 staining of the NHERF-2 shRNA–bearing hemangioma. Arrows indicate positive Ki67 (brown) staining. Original magnification, ×40.
