Overexpression of NHERF-2 arrests cell proliferation. HUVECs were transfected with 2 μg of EV or NHERF-2 plasmid with the use of nucleofection for 48 hours. Next, equal number of trypsinized cells were plated in triplicate in 6-well plates and after 24 hours were either (A) counted with trypan blue dye or (B) lysed and subjected to Western blot analysis with the use of Abs against c Myc, NHERF-2, p27, and β actin. Statistical significance was determined with 2-sided Student t test, and a value of P < .05 was considered significant.