HSC engraft onto extramedullary bones. (A) Human cord blood mononuclear cells (hMNC-CB) were injected into mice with established extramedullary bones. Mice were killed 4 weeks and 11 weeks after transplantation. Mononuclear cells were harvested from peripheral blood, femurs, and extramedullary bones, and then they were analyzed for human CD45 by flow cytometry. (B) Histograms showed the human CD45 percentage of individual mouse. (C) Histologic sections of femur (i) and extramedullary bones (ii) 4 weeks after transplantation were stained with anti–human CD45 antibody (green). (D) Femur (i) and extramedullary bone (ii) slides of 11-week transplantation mice were stained with anti–human CD19 and CD33. The figures show 1 representative result of 5 experiments.