The WS1.6-huWASp LV results in minimal B-cell correction. Analysis of WASp expression, B-cell development and function in B cells from MND versus WS1.6 recipients. (A) Pooled flow cytometry data showing WASp expression for different splenic B-cell subsets. (B) Absolute numbers of splenic B-cell subsets in different recipients. (C) BrdU uptake in splenic MZ B cells from different recipients. (D) Analysis of λ LC usage within the splenic FM and MZ B-cell subsets. (E) Absolute numbers of spontaneous GC B cells (FAS⁺PNA⁺) and autoimmune prone B cells (CD11c⁺FAS⁺) within the spleen. (F) The titers of anti–double-stranded (ds) DNA antibodies were analyzed ∼ 22 weeks after transplant at a 1:200 dilution. (G) For in vitro isotype switching, B cells were isolated and stimulated with LPS for 5 days. The numbers shown reflect percent of IgG2b⁺ B cells after dead cell exclusion. (H) Number of viral integrations within total spleen or BM was calculated using real-time PCR. Data shown in panels A, B, D, and E are based on 5 unique experiments, (n = 11 for WTM, 11 for KOM, 20 for MND and 21 for WS1.6). Anti-DNA ELISA data in panel F are based on 8 unique experiments (n = 17 for WTM, 16 for KOM, 40 for MND, and 21 for WS1.6). Data in panels C and G are based on 3 experiments, n = 6 for WTM, 6 for KOM, 12 for MND, and 13 for WS1.6. Error bars represent SD (*P < .05; **P < .01; ***P < .001).