The WS1.6-huWASp LV partially restores WASp expression and functionality in T cells. WASp expression was analyzed 20 weeks after transplant in MND- versus WS 1.6-huWASp LV recipients. (A) Representative flow cytometry plots showing WASp expression within different PB subsets. Percent WASp⁺ cells in each subset is shown in the top right corner and the MFI of WASp⁺ cells is shown in the top left corner. Pooled PB data are shown in panel B. (C) Representative flow cytometry analysis showing WASp expression in CD4⁺ splenic T-cell subsets. For T-cell functional assays, total splenocytes were stimulated with αCD3 or PMA/ionomycin and proliferation (D) and IL2 production (E) were analyzed as described in Figure 2. As the proliferation and IL2 production were similar for mock treated and nontransplanted controls, these 2 groups were combined for panels D and E to improve statistical power. Except for panel B, the data represent 2 unique experiments, n = 5 for WTM, 5 for KOM, and 8 each for MND and WS1.6. Data shown in panel B represents 5 unique experiments, n = 11 for WTM, 11 for KOM, 20 for MND, and 21 for WS1.6. Error bars represent SD (*P < .05; **P < .01; ***P < .001).