Development of myeloid clonal expansion in a single MND-huWASp LV recipient. (A) Total white blood cell numbers (top) and number of peripheral blood lin^neg cells (bottom) at various times after transplant. (B) Absolute numbers of hematopoietic subsets in the spleen and the BM 28 weeks after transplant. (C) WASp staining within distinct splenic hematopoietic subsets. (D) LAM-PCR analysis of total splenocytes and total BM from primary and secondary recipients. (E) Atf7ip mRNA expression in BM and spleen isolated from primary recipients. Error bars represent SD based on 3 separate PCR experiments.