The MND-huWASp LV transduces long-lived hematopoietic stem cells. (A) Representative flow cytometric WASp expression in PB leukocytes from primary or secondary recipients. The primary recipient in top panel was used to reconstitute secondary recipient in the bottom panel. (B-D) Summary of WASp expression in primary versus secondary recipients for splenic total hematopoietic populations (B); T-cell (C) and B-cell (D) subsets. The dotted line represents proportion of WASp⁺ monocytes in secondary recipients. (E) Number of viral integrations within total spleen or BM was calculated using real-time PCR. (F) Kaplan-Meier curves were generated on the basis of overall animal survival during the nearly 2-year follow-up period including both primary and secondary recipients. Error bars represent SD based on 19 primary and 34 secondary recipients (*P < .05; **P < .01; ***P < .001).