The MND-WASp LV mediates selection of WASp⁺ T cells and restoration of T-cell functionality. (A) WASp expression in the splenic CD11b⁺ monocyte and thymic single-positive T-cell compartment. The dotted line represents WASp expression within splenic monocyte compartment, whereas stars identify significant deviations from monocyte marking. (B) Cumulative data showing the percentage WASp⁺ cells in distinct CD4⁺ splenic T-cell subsets. (C) Representative flow cytometry data for CD4⁺ splenic subsets. The proportion of WASp⁺ cells is listed in the top right corner, whereas MFI of WASp⁺ cells is shown in the top left corner. (D) WASp expression in the BM and splenic CD3⁺NK1.1⁺ NKT compartment. (E) MFI values were obtained from WASp⁺ cells. The aggregate MFI values for splenic monocytes and CD4⁺ T cells are shown. (F) Total splenocytes were stimulated with αCD3 or PMA/Ionomycin and proliferation was quantified using thymidine incorporation. Stimulation index (SI) is the ratio of stimulated compared with unstimulated samples. (G) Total splenocytes were stimulated with αCD3 or PMA/Ionomycin for 6 hours and percent of IL2⁺CD4⁺ cells quantified by flow cytometry. With the exception of the MFI data, these findings represent 4 unique experiments with error bars indicative of SD (n = 7 for WTM, 6 for KOM, and 19 for MND). The MFI analysis is derived from 2 unique experiments, n = 4 for WTM mice and 8 for MND animals. Unless specifically indicated, stars indicate significant differences in WASp expression compared with splenic CD11b⁺ monocytes (*P < .05; **P < .01; ***P < .001).